INOCULATION AND ISOLATION TECHNIQUES IN BACTERIOLOGY    

Authors : Gautam Panwar; Ankur Vashishtha; Zeba; Gurdeep Panwar

Publishing Date : 2023

DOI : https://doi.org/10.52458/9789388996846.nsp2023.eb.ch-15

ISBN : 978-93-88996-97-6

Pages : 113

Chapter id : GU/NSP/EB/CMDT/2023/Ch-15

Abstract : Inoculation and isolation techniques are the foundation of bacteriology, enabling researchers, clinicians, and scientists to work with pure bacterial cultures, leading to accurate identification and further study. This comprehensive guide delves into the critical aspects of these techniques, covering aseptic practices, various inoculation and isolation methods, and their significance in microbiology research, clinical diagnostics, and industrial applications. The chapter discusses advanced techniques, including filtration and automation, highlighting their role in high-throughput bacterial isolation. By mastering these fundamental procedures, practitioners can ensure the integrity of bacterial cultures and lay a solid foundation for precise and insightful bacterial studies.

Keywords : Inoculation techniques, Isolation techniques, Aseptic techniques, Bacterial cultures.

Cite : Panwar, G., Vashishtha, A., Z., & Panwar, G. (2023). Inoculation And Isolation Techniques In Bacteriology (1st ed., p. 106). Noble Science Press. https://doi.org/10.52458/9789388996846.nsp2023.eb.ch-15

References :
  1. American Society for Microbiology. (2019). Basic Protocol 2: Inoculation of Media. In "Manual of Microbiological Culture Media." ASM Press.
  2. Atlas, R. M. (2010). Principles of microbiology. Jones & Bartlett Publishers.
  3. Cappuccino, J. G., & Sherman, N. (2018). Microbiology: A Laboratory Manual (12th ed.). Pearson.
  4. Collins, C. H., Lyne, P. M., & Grange, J. M. (2007). Microbiological Methods (8th ed.). Butterworth-Heinemann.
  5. Cowan, S. T., & Steel, K. J. (1974). Manual for the identification of medical bacteria. Cambridge University Press.
  6. Forbes, B. A., Sahm, D. F., & Weissfeld, A. S. (2007). Bailey & Scott's Diagnostic Microbiology (12th ed.). Mosby.
  7. Janeczko, L., & Iwaniak, W. (2018). Automation in microbiology. Advances in Clinical Chemistry, 86, 1-32. doi: 10.1016/bs.acc.2018.08.002
  8. Kabiraz, M. P., Majumdar, P. R., Mahmud, M. C., Bhowmik, S., & Ali, A. (2023). Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review. Heliyon.
  9. Prescott, L. M., Harley, J. P., & Klein, D. A. (2023). Microbiology (12th ed.). McGraw-Hill Education.
  10. Stellrecht, K. A., & Hardy, D. J. (2003). Automation and nucleic acid amplification testing in the diagnostic microbiology laboratory. Clinical Chemistry, 49(10), 1763-1767. doi: 10.1373/49.10.1763
  11. Tortora, G. J., Funke, B. R., & Case, C. L. (2016). Microbiology: An Introduction (12th ed.). Pearson.