Abstract : Gram staining is a fundamental microbiological technique developed by Hans Christian Gram in the 1880s. This chapter provides a detailed exploration of the method, encompassing its principles, procedure, interpretation, and significance in microbiology. It highlights the differences between Gram-positive and Gram-negative bacteria, offers insights into common errors, and discusses the mechanism behind staining outcomes. The chapter concludes by showcasing examples of bacteria from each category, underscoring the enduring importance of Gram staining in microbiological research and diagnostics.
Cite : Panwar, G., Panwar, G., & Bharti, T. (2023). Gram Staining: A Fundamental Technique In Microbiology (1st ed., p. 106). Noble Science Press. https://doi.org/10.52458/9789388996846.nsp2023.eb.ch-14
References :
Cappuccino, J. G., & Sherman, N. (2018). Microbiology: A Laboratory Manual (12th ed.). Pearson.
Madigan, M. T., Martinko, J. M., Bender, K. S., Buckley, D. H., & Stahl, D. A. (2018). Brock Biology of Microorganisms (15th ed.). Pearson.
O'Toole GA. Classic Spotlight: How the Gram Stain Works. J Bacteriol. 2016 Dec 01;198(23):3128. [PMC free article] [PubMed]
Pelczar, M. J., Chan, E. C. S., & Krieg, N. R. (2022). Gram staining. In Microbiology (7th ed., Tata McGraw-Hill Education.
Prescott, L. M., Harley, J. P., & Klein, D. A. (2023). Microbiology (12th ed.). McGraw-Hill Educatio
Tortora, G. J., Funke, B. R., & Case, C. L. (2016). Microbiology: An Introduction (12th ed.). Pearson.
Tripathi, N., & Sapra, A. (2023, August 14). Gram Staining. In StatPearls [Internet]. Treasure Island, FL: StatPearls Publishing. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK562156/.