CHROMATOGRAPHY BASED DNA ISOLATION METHODS    

Authors : Ashrita Dubey; Sheikh Abdul Wase

Publishing Date : 2023

DOI : https://doi.org/10.52458/9789388996846.nsp2023.eb.ch-09

ISBN : 978-93-88996-97-6

Pages : 61

Chapter id : GU/NSP/EB/CMDT/2023/Ch-09

Abstract : Since Friedrich Miescher's ground breaking work in 1869, DNA extraction methods have undergone remarkable progress. Initially, liquid-liquid extraction using phenol-chloroform paved the way for DNA research but came with drawbacks, such as the use of toxic chemicals. In response, safer alternatives, including physical extraction methods like magnetic beads and cellulose-based filter paper, have revolutionized the field. With the rise of gene editing and personalized medicine, there is a heightened demand for reliable DNA isolation techniques. Researchers and clinicians now seek methods that consistently yield high-quality DNA with minimal impurities for applications like gene therapy and disease diagnosis. The evolution of DNA extraction methods reflects the dynamic nature of scientific research, emphasizing the continuous pursuit of improvement and innovation in molecular biology. As technology advances and research needs evolve, we anticipate the emergence of even more efficient and sophisticated DNA extraction techniques. These advancements will play a crucial role in propelling scientific discoveries and furthering personalized medicine.

Keywords : DNA Extraction, DNA, Phenol –Chloroform, Chromatography Based DNA Extraction

Cite : Dubey, A., & Wase, S. A. (2023). Chromatography Based Dna Isolation Methods (1st ed., p. 61). Noble Science Press. https://doi.org/10.52458/9789388996846.nsp2023.eb.ch-09

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