Abstract : In the dynamic landscape of genetic research and personalized medicine, the demand for high-quality genomic DNA has intensified. Traditionally sourced from blood, the need for less invasive, cost-effective, and user-friendly DNA collection methods is evident. Addressing this, we present a novel protocol utilizing commercial mouthwash for extracting premium genomic DNA from accessible buccal cells. Participants swish undiluted mouthwash, followed by DNA extraction involving proteinase K digestion, phenol-chloroform extraction, and ethanol precipitation. The result is genomic DNA of superior quality and quantity, suitable for diverse molecular analyses. Validating efficacy, a study with 60 subjects successfully genotyped participants for various genetic polymorphisms using PCR-based assays. Crucially, our protocol allows storage of unprocessed specimens at room temperature or -20°C for extended periods without compromising DNA quality or PCR amplifiability. This method holds promise for large community-based genetic susceptibility studies, enabling participants to self-collect, mail, and store samples. Its scientific rigor, cost-effectiveness, and reliability open transformative avenues in genetic research and beyond.
Keywords : DNA Extraction, Buccal Cells, PCR, PCR amplifiable, Re-suspension, DNA isolation, Phenol-chloroform, DNA Pellet.
Cite : Dubey, A., Wase, S. A., Panwar, G., Singh, N., & Pal, S. (2023). Comprehensive Buccal Mucosal Epithelial Cell Dna Extraction: Techniques And Applications (1st ed., p. 55). Noble Science Press. https://doi.org/10.52458/9789388996846.nsp2023.eb.ch-08
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